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Review of 9th Annual Symposium |
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The 9th Annual Symposium on Advances in Separation Science and Mass Spectrometry was a great success!
9th Annual Symposium on Advances in Separation Science and Mass Spectrometry at Northeastern University Wednesday May 9, 2007 Organized by MASSEP.org and the Greater Boston Mass Spectrometry Discussion Group (GBMSDG) Separations Speaker: Professor Robert Kennedy (University of Michigan Department of Chemistry)(click here for speaker biography)
Title: "High-Throughput Separations" (click here for abstract)
Mass Spectrometry Speaker: Professor Julie Leary, (University of California Davis Department of Chemistry)(click here for speaker biography) Title: "Advancing the Frontiers of Structural Biology; Mass Spectrometry of Biological Binding Partners" (click here for abstract) Link to Poster Abstracts Poster Contest Winner Separations Speaker Abstract: Title: "High-Throughput Separations", Robert T. Kennedy, Hobart H. Willard Professor of Chemistry & Professor of Pharmacology, University of Michigan Abstract: Over the past decade miniaturized electrophoresis techniques have become high-throughput analysis tools. This has been made possible by increasing the speed of separations through utilization of high-electric fields on short separation distances, operating separations in parallel arrays, or the combination of these strategies. Further increases in throughput have been made possible by microfluidics, which have made it possible to integrate more complex sample manipulations with high-throughput electrophoresis. Prominent applications of high throughput electrophoresis have been genome sequencing and drug characterization. In our laboratory, we have investigated use of high-speed and parallel separations for: 1) monitoring brain chemistry dynamics in vivo; 2) monitoring the chemical dynamics of living cells; and 3) for drug discovery screening. We will discuss these applications and the instrumentation that makes them possible. These results demonstrate that high-throughput separation methods have the potential to impact many bioanalytical areas. Biography: Robert Kennedy is the Hobart H. Willard Professor of Chemistry and a Professor of Pharmacology at the University of Michigan-Ann Arbor. His research interests are in the area of analytical chemistry and its application to pharmacology. His group has pioneered the use of capillary electrophoresis (CE), capillary liquid chromatography (LC) and microelectrodes for the study of neurotransmitters and hormones at single living cells and in vivo. An important accomplishment was the development of a microelectrode that allowed insulin release to be monitored at single pancreatic beta cells. This method has since been used to study the mechanisms of secretion and its relationship to diabetes. More recently, a method for imaging secretion based on confocal fluorescence microscopy has been developed. The Kennedy group has also developed instrumentation that couples in vivo sampling methods with CE. This technique has allowed significant improvements in the in vivo detection of neurotransmitters. This development has opened the door to numerous studies on the chemical substrates for behavior. As part of this project, the group invented the CE-based immunoassay and a variety of other affinity based methods which have been used in clinical as well as basic research applications. More recently his group has developed a highly sensitive LC-MS method that has allowed neuropeptides to be monitored and discovered in the brain of living rats. Professor Kennedy earned a B.S. in Chemistry at the University of Florida in 1984 and a Ph.D. from the University of North Carolina in 1988. After a two year stint as a NSF post-doctoral fellow, also at North Carolina, he served as a professor of chemistry at the University of Florida for 11 years. He has served on the editorial board of several journals including the Journal of Chromatography, The Analyst, and Electrophoresis. He has received several awards including the Presidential Faculty Fellowship, ACS Findeis Award, Benedetti-Pichler Award of the Microchemical Society, NSF National Young Investigator Award, Beckman Young Investigator Award, Lilly Analytical Research Award, College of Liberal Arts and Sciences Teacher of the Year Award, and Alfred P. Sloan Fellowship. Abstract: Advancing the frontiers of Structural Biology; Mass Spectrometry of Biological Binding Partners; Professor Julie Leary, Departments of Chemistry and Molecular and Cellular Biology, and the UC Davis Genome Center and Bioinformatics Program, University of California Davis Complex Biological systems, such as protein:protein and protein:carbohydrate interactions, are important in cell communication and signaling events. Until recently, mass spectrometry has been relegated to small molecule analysis. However, with the advent of new ionization methods and mass analyzers, analysis of non-covalent complexes is now possible. This lecture will cover protein:carbohydrate binding partners involved in chemotaxis as well as characterization of the thirteen subunit complex comprising the eukaryotic initiation factor, EIF3. Mass and ion mobility analysis provide important information on topology and specificity of these binding partners. Biography: Professor Julie A. Leary received her Ph.D. at MIT under the mentorship of Prof. Klaus Biemann. After a combined postdoctoral position at Woods Hole Oceanographic and MIT , Dr. Leary took a position as Director of Analytical Facilities at U.C. Berkeley where she held the position of adjunct professor. In 2004 she became Prof. of Molecular and Cellular Biology and Chemistry at U.C. Davis. Prof. Leary is the recipient of several awards, among them is the prestigious Biemann medal awarded in 2000, given to investigators who have made significant contributions to the field of mass spectrometry early in their careers. Prof. Leary is on the editorial boards of Glycobiology, JASMS, and the International Journal of Mass Spectrometry. She has held the position of Member at Large for Education on the board of the American Society of Mass Spectrometry and currently sits on several executive boards including that for the Complex Carbohydrate Research Center at the University of Georgia. |
